Recent paper: Core Genome Multi Locus Sequence Typing and Single Nucleotide Polymorphism Analyses in the Epidemiology of Brucella melitensis Infections
Keywords :
Authors
Anna Janowicz, Fabrizio De Massisa, Massimo Ancoraa, Cesare Cammàa, Claudio Patavinoa, Antonio Battistib, Karola Priorc, Dag Harmsenc, Holger Scholzd, Katiuscia Zillia, Lorena Sacchinia, Elisabetta Di Giannatalea and Giuliano Garofolo
Abstract [relayed from jcm.asm.org]
The use of whole genome sequencing (WGS) using next generation sequencing (NGS) technology has become a widely accepted method for microbiology laboratories in the application of molecular typing for outbreak tracing and genomic epidemiology. Several studies demonstrated the usefulness of WGS data analysis through single nucleotide polymorphism (SNP) calling from a reference sequence analysis for Brucella melitensis, whereas gene-by-gene comparison through core-genome multilocus sequence typing (cgMLST) has not been explored so far. The current study developed an allele-based cgMLST method and compared its performance to the genome-wide SNP approach and the traditional MLVA on a defined sample collection. The dataset comprised of 37 epidemiologically linked animal cases of brucellosis as well as 71 isolates with unknown epidemiological status composed of human and animal samples collected in Italy.
New! LEILA, a software to simplify organisation of Inter-Laboratory Proficiency tests !
Dear colleagues,
Anses has developed a software/web interface (https://leila.anses.fr/) to simplify organisation of Inter-Laboratory Proficiency tests !
This software will facilitate the access to all the information concerning the ILPT (calendar, online registration, documents, reports).
Each laboratory has now to create a Laboratory workspace into the software. You can find a tutorial to create your workspace (https://vimeo.com/ansesleila).
After this creation, you will be ready for the future ILPT.
Hope to see you soon on LEILA!
In case of any further question, please contact us (eurl.brucellosis@anses.fr)
Successful first training session on biological controls at ANSES !
The EURL team organised the first 2018 training session on the 14th and 15th June at ANSES. This 2-day training session focused on control of ELISAs and Rose Bengale (RB) antigens. Eight participants from Croatia, Ireland, Lithuania, Malta, Portugal, Romania, Slovenia and Spain performed a successful control of a serum ELISA kit and two RB antigens. It was a good opportunity to exchange on critical points of reagents controls and share experience on protocols and reagents.