URL: 
https://eurl-brucellosis.anses.fr
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color
Display Name: 
Brucellosis
Title [EN]: 
Methods
Methods Background: 
Materiau Background: 

Milk Ring Test (MRT)

(cattle only)

The present document describes a technique aiming at detecting antibodies specific of smooth Brucella species (B. abortus, B. melitensis and B. suis) in bovine milk from the bulk tank by the Milk Ring-Test (MRT).

In lactating cattle, the MRT can be used for screening herds for brucellosis. In large herds (> 100 lactating cows), the sensitivity of the test becomes less reliable.

False-positive reactions may occur in cattle vaccinated less than 4 months prior to testing, in samples containing abnormal milk (such as colostrum) or in cases of mastitis.

Download the method

2021 Brucellosis Specifications for validation of Antigen for Complement Fixation Test

The present document describes the requirements for validation (fitness for purpose) of antigen for complement fixation test (CFT) in brucellosis diagnostic. The standardisation and validation are critical steps when a method is intended for routine diagnostic use in multiple laboratories. Therefore, customers can have confidence in the results produced by the test. All diagnostic assays should be validated for the species which they will be used for, according to the new OIE and EU principles and methods.

English

2021 Brucellosis Milk Ring-Test

The present document describes a technique aiming at detecting antibodies specific of smooth Brucella species (B. abortus, B. melitensis and B. suis) in bovine milk from the bulk tank by the Milk Ring-Test
(MRT).

English

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