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Enumeration

Published on 10/04/2015

Official Reference Standard Method EN ISO 6888-1:1999/A1:2003 EN/ISO 6888-2:1999/A1:2003 Proficiency tests dedicated to the enumeration of Coagulase Positive Staphylococci 2018: dispatch of sample 28 May 2018, analysis to be performed on 4 June 2018, results to be transmitted by 12 June 2018 (cover letter, thermotrack instructions) 2017: final report, follow up 2015-2017 2016: final report, follow up 2014-2016 2015: final report, follow up 2013-2015 2014: final report, follow-up 2012-2014 2013: final report   Inoculation techniques 2015: study with cheese -  report 2011-2014: study with shelled cooked prawns - report 2012-2013: study on powdered infant food formulae   Measurement uncertainty Study on the heterogeneity of CPS contamination Impact of the size of the test portion: - report of the comparison 10vs100g - report on 10vs25g comparison    

Characterisation

Published on 03/04/2015

Characterisation methods Conventional multiplex PCR (sea to see and ser and seg to sej and sep) Multiplex RT-PCR (soon) PFGE: EURL method (2010) Training courses Conventional multiplex PCR: - next course: 19-20 November 2015, invitation - registration - last course: 2014 PFGE: - last course: 5-7 November 2013 (4 participants) Analytical development Validation of a multiplex real-time (RTi) PCR method for se genes detection For the investigation of staphylococcal food-borne outbreaks (SFPOs), it is often necessary to detect the presence of se genes in CPS strains, in addition to SE detection in the suspected food, in order to confirm the identity of the food responsible for SFPO. EURL CPS has been developing a multiplex real-time (RTi) PCR scheme for the detection of 13 se genes. A validation trial is on going, results are expected for the 15 December 2015. The method expected to be dispatch in 2016. Molecular alternative methods to PFGE  1. spa-typing Internal method available - please contact the EURL for further details 2. MLVA This was a Multi Locus Variable number tandem repeat (VNTR) Analysis (MLVA), coupled with capillary electrophoresis (CE). 16 VNTR loci have been selected, including those described by Pourcel et al (Journal of Clinical Microbiology, 2009, 3121-3128). This protocol was tested on a large panel of strains including reference and field food strains. The results obtained showed that MLVA was as discriminatory as PFGE. 3. NGS Method to be assessed compared to PCR, PFGE, MLVA and spa-typing. Work for 2016-2017: bibliographic study and comparison with a batch of well characterised strains. Technical assistance to NRLs EURL CPS will ensure scientific and technical assistance for the characterization of CPS strains (detection of se genes and typing of CPS strains) for NRLs upon request. - Analysis at the charge of the EURL; - Transport of samples at the charge of the NRL Samples should be sent to Frederic.AUVRAY (ACI-MON-017) - Anses - 22, rue Pierre et Marie Curie 94701 Maisons-Alfort cedex Dispatch of strains Upon request of the NRLs and in order to implement methods for detection of se genes and for sub-typing of CPS strains, EURL CPS would send CPS field strains and/or DNA material from its collection to the NRLs.  

Extraction by dialysis concentration step

Published on 29/01/2015

Step by step

Working areas

Published on 22/10/2014

Bacterial Detection enumeration (soon) Staphylococcal enterotoxins Characterisation (soon)

Staphylococcal enterotoxins

Published on 22/10/2014

European Screening Method for SE detection European screening method v5, September 2010 - pdf Extraction by dialysis concentration step - video Training courses dedicated to SE analysis Horizontal Standard method for the immunoenzymatic detection of staphylococcal enterotoxins in foodstuffs (EN ISO 19020:2017): next course: invitation, training programme, registration   European screening method v5, September 2010: last course: 19-20 September 2016                            Quantitative ELISA: last course: 2014 Proficiency testing trials dedicated to SE detection in food European screening method v5, September 2010: 2017 - results received, preliminary reports sent in May, report expected in September 2016 - final report / PT-followup 2015 - final report / PT-followup 2014 - final report 2013 - 2nd version of the final report     Technical assistance to NRLs In the case of staphylococcal food poisoning outbreak, EURL could perform the confirmatory analysis for NRLs. Analysis at the charge of the EURL Transport of samples at the charge of the NRL Samples should be sent to Yacine NIA (ACI-MON-017) - Anses - 22, rue Pierre et Marie Curie 94701 Maisons-Alfort cedex New certified reference materials to detect staphylococcal enterotoxin A in cheese Reference materials (IRMM-359a-c) can be ordered directly from the JRC-IRMM or from one of the authorised distributors. - Certification report, - List of authorised distributor in page vi of the RM catalogue (2016-05-04), - Price list (2016-05-04). References: - Development of a reference material for Staphylococcus aureus enterotoxin A in cheese: Feasibility study, processing, homogeneity and stability assessment (2015) doi:10.1016/j.foodchem.2014.07.066 - Certified reference materials for testing of the presence/absence of Staphylococcus aureus enterotoxin A (SEA) in cheese (2016) DOI: 10.1007/s00216-016-9642-5    

Accommodation in Maisons-Alfort

Published on 24/09/2014

Prior to book your hotel we invite you to visit the useful information page to see how you can get to our laboratory. We propose you to stay in one of these hotels by order of recommendation: Kyriad Paris-Est Porte de Bercy, le Saint Maurice, 13 min walking from the laboratory - link Adagio, 9 min walking from the laboratory - link Le Petit Caporal, 7 min walking from the laboratory - link

Eligibility rules

Published on 22/09/2014

In order to facilitate the reimbursement of your travel and stay costs, please find hereafter the link to the procedure: https://eurl-staphylococci.anses.fr/en/minisite/staphylococci/instructions-reimbursement-travel-costs-and-daily-allowances-0 Main steps to follow: - As soon as possible book your flight or train; - 15 days before the event the travel expense form to be filled out and send by EMAIL : Financial Identification Form (FIF)  with copy of your travel tickets stating the price and the invoice of the travel agency; - during the event provide ORIGINAL documents: FIF, boarding pass, train/flight tickets and invoice, hotel bill if relevant; - 15 days after the event by MAIL: ORIGINAL return boarding pass/train ticket and balanced hotel bill.

PT trials dedicated to SE detection

Published on 23/07/2014

2014 preliminary report   2013 report satisfaction enquiry outcome

News calendar

Published on 18/07/2014

News, calendar of events, information News and events Workshops & Meetings Slide shows of 23-25 May 2012 general workshop at Valetta (Malta) are available. All workshop information Proficiency testing trials Week 47 (between the 19th and the 23rd of November, 2012) Enumeration of coagulase positive staphylococci in powdered infant formulae Reports Trial on the detection of staphylococcal enterotoxins, PT trial 2011 Trial on the detection of staphylococcal enterotoxin genes by PCR, PT trial 2010    All proficiency tests information Training session 11-12 September 2012 Principle and the technical practice on the ESM for the detection of SE types SEA to SEE in all types of food matrices 09-12 July 2012 28-29 August 2012 Principle and the EURL quantitative detection method by ELISA   All training session information